Polímeros: Ciência e Tecnologia
Polímeros: Ciência e Tecnologia
Scientific & Technical Article

Characterization of the Galactomannans from Parkinsonia Aculeata Seeds and their Application on Affinity Chromatography

Moreira, Renato A.; Sierakowski, Maria Rita; Petkowicz, Carmen L. O.; Reicher, Fany; Garros-Rosa, Ivone

Downloads: 0
Views: 617


Successive aqueous (10 °C, 25 ºC and 80 °C) and alkaline (1M NaOH; 25 °C) extractions of milled Parkinsonia aculeata endosperms gave rise to four galactomannan fractions. These extractions furnished viscous galactomannans with Man:Gal ratios ranging from 3.1:1; 3.7:1; 4.9:1 and 6.1:1 (P1, P2, P3 and P4, respectively). Fraction P1 was used for structural studies by using methylation analysis, periodate oxidation and 13C-NMR. It showed a linear backbone of β(1→4) linked D-mannose units, to which single α(1→6)-linked D-galactose are attached. This galactomannan has Mw 775700 g/mol and intrinsic viscosity of 558 mL/g. The four fractions and the crude endosperm were treated with epichlorydrin and used as matrix for affinity chromatography. All columns tested showed ability to bind lectin samples. The efficiency is related to the degree and pattern of substitution of galactosyl units on the D-mannan backbone.


Galactomannan, Parkinsonia aculeata, lectin, affinity chromatography


1. Dea, I. C. M. & Morrison, A. Adv. Carbohydr. Chem. Biochem., 31, p.241 (1975).

2. Reid, J. S. G. & Edwards, M. E. “Galactomannans and other cell wall storage polysaccharides in seeds” In: Food Polysaccharides and their Applications. p.155-186, Stephen, A. M. (ed) Marcel Dekker, Inc. New York (1995).

3. Mccleary, B.V.; Taravel, F.R. & Cheetham, N.W.H. Carbohydr. Res., 104(2), p.285 (1982).

4. McCleary, B.V.; Nurthen, E.; Taravel, F.R. & Joseleau, J.P. Carbohydr. Res., 118, p91 (1983).

5. Ganter, J. L. M. S.; Heyraud, A.; Petkowicz, C. L. O.; Rinaudo, M.& Reicher, F. Int. J. Biol. Macromol., 17(1), p.13 (1995).

6. Petkowicz, C. L. O.; Sierakowski, M-R.; Ganter, J. L. M. S. & Reicher, F. Phytochemistry, 49(3), p.737 (1998).

7. Petkowicz, C. L. O.; Reicher, F. & Mazeau, K. Carbohydr. Polym., 37, p.25 (1998).

8. Petkowicz, C. L. O.; Milas, M.; Mazeau, K.; Bresolin, T.; Reicher, F. Ganter, J. L. M. S. & Rinaudo, M. Food Hydrocolloids, 13, p. 263 (1999).

9. Dolník, V.; Gurske, W.A. & Padua, A. Electrophoresis, 22(4), p.707 (2001).

10. Figureueiró, S.D.; Góes, J.C.; Moreira, R.A. & Sombra, A.S.B. Carbohydr. Polym., 2004 (in press)

11. Moreira, R.A; Castelo-Branco, C.C.; Monteiro, A.C.O.; Tavares, R. & Beltramini, L. Phytochemistry, 47(7), p. 1183 (1998).

12. Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebers, P. A. & Smith, F. Anal. Chem., 28, p.350 (1956).

13. Baethgen, W.M. & Alley, M.M. Commun. Soil Sci. Plant Anal., 20 (9/10), p.961 (1989).

14. Hartree, E. F. Anal. Biochem., 48, p.422 (1972).

15. Bradford, M. M. Anal.Biochem., 72, p.248 (1976).

16. Trevelyan, W. E.; Procter, D. P. & Harrison, J. S. Nature, 166, p.444 (1950).

17. Ciucanu, J. & Kerek, F. Carbohydr. Res., 131, p. 209 (1984).

18. Ganter, J. L. M. S.; Zawadzki-Baggio, S. F.; Leitner, S. C. S.; Sierakowski, M. R. & Reicher, F. J. Carbohydr. Chem., 12(6), p.753 (1993).

19. Appukutan, P.S.; Surolia, A. & Bachhawat, B.K. Indian J. Biochem. Biophys. 14, p.382 (1977).

20. Moreira, R.A & Perrone, J.C. Plant Physiology, 59, p.783 (1977).

21. Laemmli, U.K. Nature, 227, p.680 (1970).

22. Manzi, A. E.; Mazzini, M. N. & Cerezo, A. S. Carbohydr. Res., 125, p.127 (1984).

23. Gurha, S.G. & Singh, L. Carbohydr. Res., 182, p.144 (1988).

24. Grasdalen, H. & Painter, T. Carbohydr. Res., 81, p.59 (1980).

25. Garros-Rosa, I. – “Galactomananas de Parkinsonia aculeata L.: caracterização estrutural e aplicação no isolamento de lectinas ligantes de galactose”, Tese de doutorado, Universidade Federal do Ceará, Brasil (2000).

26. Tavares, R.O. - “Galactomanana de Adenanthera pavonina L. Aplicação para o isolamento de lectinas galactoseespecíficas”, Dissertação de Mestrado, Universidade Federal do Ceará, Brasil (1988).

27. Ganter, J.L.M.S.; Cardoso, A.T.M.; Kaminski, M. & Reicher, F. Int. J. Biol. Macromolecules, 21, p.137 (1997).

28. Reicher, F.; Leitner, S. C. S.; Sierakowski, M. R.; Fontana, J. D. & Correa, J. B. C. Appl. Biochem. Biotechnol., 28/29, p.353 (1991).
5883710d7f8c9d0a0c8b46de polimeros Articles
Links & Downloads

Polímeros: Ciência e Tecnologia

Share this page
Page Sections